Supporting Information Direct Observation of Growth and Shrinkage of Microtubules by Single Molecule Förster Resonance Energy Transfer
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چکیده
1.1 Materials: Diamino-polyethylene glycol with MW 2000 Da (PEG2000) was purchased from Rapp Polymer. (3-Glycidoxypropyl)trimethoxysilane (GOPTS) was purchased from Fluka. EZ-Link NHSBiotin was purchased from Thermo Scientific. Hydrogen peroxide (30% solution), Acetone, Dichloromethane and N, N-Dimethylformamide were purchased from Merck. Imidazole and 2-[4-(2hydroxyethyl) piperazin-1-yl]ethanesulfonic acid (HEPES) were purchased from Himedia. 1,4Piperazinediethanesulfonic acid (PIPES), ethylene-bis(oxyethylenenitrilo)tetraacetic acid (EGTA), guanosine-5 ́-triphosphate sodium salt hydrate (GTP), β-casein, catalase, glucose oxidase and βmercaptoethanol were purchased from Sigma Aldrich. Alexa Fluor 568 carboxylic acid succinimidyl ester was purchased from Invitrogen. Guanosine-5’-[(α,β)-methyleno]triphosphate Sodium salt (GMPCPP) was purchased from Zena Bioscience.
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